Journal
METHODS
Volume 68, Issue 1, Pages 218-227Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2014.04.020
Keywords
Drosophila; Spermatogenesis; Germ line; Stem cell; Meiosis; Cytokinesis
Funding
- South-Eastern Norway Regional Health Authority
- Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research at the University of California - Los Angeles
- NIH
- CIRM
- Norwegian Cancer Society
Ask authors/readers for more resources
The process of spermatogenesis in Drosophila melanogaster provides a powerful model system to probe a variety of developmental and cell biological questions, such as the characterization of mechanisms that regulate stem cell behavior, cytokinesis, meiosis, and mitochondrial dynamics. Classical genetic approaches, together with binary expression systems, FRT-mediated recombination, and novel imaging systems to capture single cell behavior, are rapidly expanding our knowledge of the molecular mechanisms regulating all aspects of spermatogenesis. This methods chapter provides a detailed description of the system, a review of key questions that have been addressed or remain unanswered thus far, and an introduction to tools and techniques available to probe each stage of spermatogenesis. (C) 2014 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available