Journal
METHODS
Volume 67, Issue 1, Pages 55-63Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2013.07.014
Keywords
Genome-wide technology; RNA degradation; RNA decay; RNA stability; High-throughput sequencing; Massive sequencing analysis
Funding
- Suzuken memorial foundation
- Naito Foundation
- Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT) [221S0002]
- Japan Society for the Promotion of Science
- Grants-in-Aid for Scientific Research [24115706] Funding Source: KAKEN
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We recently developed a novel transcriptome analysis method, termed 5'-bromo-uridine (BrU) immunoprecipitation chase-deep sequencing analysis (BRIC-seq). BRIC-seq enables the determination of genomewide RNA stability by chasing chronological decreases of BrU-labeled RNAs under physiologically undisturbed conditions. The RNA half-life of each transcript is calculated from the decreasing number of BrU-labeled RNA sequence tags measured by deep sequencing of BrU-labeled RNAs. Here, we describe a detailed protocol and provide tips for BRIC-seq, followed by computational analysis. (C) 2014 Published by Elsevier Inc.
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