Journal
METHODS
Volume 61, Issue 3, Pages 260-268Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2013.03.008
Keywords
Metabolic labeling; Mass spectrometry; Quantitative proteomics; Census; Neurodevelopment
Funding
- NIH [R01 MH067880, P41 RR011823/GM103533]
Ask authors/readers for more resources
Metabolic labeling of rodent proteins with N-15, a heavy stable isotope of nitrogen, provides an efficient way for relative quantitation of differentially expressed proteins. Here we describe a protocol for metabolic labeling of rats with an N-15-enriched spirulina diet. As a case study, we also demonstrate the application of N-15-enriched tissue as a common internal standard in quantitative analysis of differentially expressed proteins in neurodevelopment in rats at two different time points, postnatal day 1 and 45. We briefly discuss the bioinforrnatics tools, ProLucid and Census, which can easily be used in a sequential manner to identify and quantitate relative protein levels on a proteomic scale. (C) 2013 Published by Elsevier Inc.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available