Journal
METHODS
Volume 58, Issue 2, Pages 164-170Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2012.07.030
Keywords
miRNA; piRNA; Deep sequencing; Digital gene expression
Funding
- Charles Revson, Jr. Foundation
- National Institute of Neurological Disorders and Stroke [NS072235]
- RUCCTS [UL1RR024143]
- NIH [MH08442]
- Starr Foundation
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The characterization of post-transcriptional gene regulation by small regulatory (20-30 nt) RNAs, particularly miRNAs and piRNAs, has become a major focus of research in recent years. A prerequisite for characterizing small RNAs is their identification and quantification across different developmental stages, and in normal and disease tissues, as well as model cell lines. Here we present a step-by-step protocol for generating barcoded small RNA cDNA libraries compatible with Illumina HiSeq sequencing, thereby facilitating miRNA and other small RNA profiling of large sample collections. (C) 2012 Elsevier Inc. All rights reserved.
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