Journal
METHODS
Volume 58, Issue 2, Pages 118-125Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2012.10.006
Keywords
Small RNA; miRNA; miR165/166; miR-30d; Short tandem target mimic (STTM); Target mimic; miRNA sponge; CeRNA
Funding
- USDA-NRI [2006-35301-17115, 2006-35100-17433]
- NSF [MCB-0718029, S-00000260, IOS-1048216/IOS-1219316]
- NIH [K01 DK078648, R03 DK084166]
- Division Of Integrative Organismal Systems
- Direct For Biological Sciences [1219316] Funding Source: National Science Foundation
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Small RNAs are widespread in plants and animals. They largely include microRNAs (miRNAs) and short interfering RNAs (siRNAs), and they play key roles in gene and chromatin regulations. Here we describe in detail the method for an effective construction of the recently developed short tandem target mimic (SUM) technology to block small RNA functions in plants and animals. SUM is a powerful technology complementing the previous target mimic (TM) in plants and the miRNA sponge, as well as the recently defined endogenous competing RNA (CeRNA) in animals. We expect SUM will not only be effective in blocking small RNA functions in plants but will also become a popular approach in animals. (C) 2012 Elsevier Inc. All rights reserved.
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