Journal
METHODS
Volume 53, Issue 1, Pages 102-109Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2010.06.014
Keywords
In vitro evolution; Recombinase; Human immunodeficiency virus type 1; Long terminal repeat; Humanized MICE; Rag2(-/-)gamma c-/-
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Current antiretroviral therapies would greatly benefit from the concurrent removal of integrated HIV-1 proviral DNA from the patient's cells. In this review, we describe an experimental strategy that allowed the engineering and functional analysis of a HIV-1 LTR-specific recombinase (Tre-recombinase). We furthermore provide protocols that are utilized for the investigation of Ire's antiretroviral activity in infected tissue cultures as well as in infected humanized Rag2(-/-)gamma c(-/-) mice. (c) 2010 Elsevier Inc. All rights reserved.
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