Journal
METHODS
Volume 50, Issue 4, Pages 298-301Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2010.01.032
Keywords
microRNA; Biomarker; Circulating; qRT-PCR; miRNA
Funding
- Department of Defense (DOD) Prostate Cancer New Investigator Award [PC074012]
- Pacific Ovarian Cancer Research Consortium Specialized Program of Research Excellence (SPORE) in Ovarian Cancer Career Development Award [NIH P50 CA83636]
- NIH/NCI Cancer Center [5 P30 CA015704]
- Pacific Northwest Prostate Cancer SPORE Pilot [NIH P50 CA97186]
- DOD Ovarian Cancer Career Development Award [00080159]
- Prostate Cancer Foundation Creativity Award
- Damon Runyon Cancer Research Foundation
Ask authors/readers for more resources
MicroRNAs (miRNAs) are small (similar to 22 nt) RNAs that play important roles in gene regulatory networks by binding to and repressing the activity of specific target mRNAs. Recent studies have indicated that miRNAs circulate in a stable, cell-free form in the bloodstream and that the abundance of specific miRNAs in plasma or serum can serve as biomarkers of cancer and other diseases. Measurement of circulating miRNAs as biomarkers is associated with some special challenges, including those related to pre-analytic variation and data normalization. We describe here our procedure for qRT-PCR analysis of circulating miRNAs as biomarkers, and discuss relevant issues of sample preparation, experimental design and data analysis. (C) 2010 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available
Share Paper
