4.7 Article

Working with OpusXpress: Methods for high volume oocyte experiments

Journal

METHODS
Volume 51, Issue 1, Pages 121-133

Publisher

ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2010.01.012

Keywords

Oocytes; High throughput; Ion channels; Heterologous expression; Drug development

Funding

  1. NIA NIH HHS [P01 AG10485, P01 AG010485] Funding Source: Medline
  2. NIDA NIH HHS [DA 017548, U19 DA017548] Funding Source: Medline
  3. NIGMS NIH HHS [R01 GM057481-08, GM57481, R01 GM057481] Funding Source: Medline

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OpusXpress is a semi-automated system for high throughput voltage clamp recording from Xeno pus oocytes. We participated in the development process for this system and were the only laboratory to field test a prototype. Subsequently, we obtained an early production model that we have used on a regular basis for the last seven years, conducting many thousands of experiments, publishing extensively, and carrying out collaborative research in drug discovery. In this article, we relate our experience with the OpusXpress recording system and large volume oocyte handling. We provide our standard operating procedures and outline the organization of our successful team. Some of our advice is specific to researchers fortunate enough to have access to an OpusXpress system, but most of it is applicable to any group using Xenopus oocytes for the heterologous expression of ion channels. (C) 2010 Elsevier Inc. All rights reserved.

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