Journal
METHODS
Volume 48, Issue 3, Pages 240-248Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2009.03.001
Keywords
ChIPseq; ChIP-seq; Chromatin immunoprecipitation; High-throughput sequencing
Funding
- Cancer Research UK [A15603, 15603] Funding Source: Medline
- European Research Council [202218] Funding Source: Medline
- European Research Council (ERC) [202218] Funding Source: European Research Council (ERC)
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Chromatin immunoprecipitation (ChIP) allows specific protein-DNA interactions to be isolated. Combining ChIP with high-throughput sequencing reveals the DNA sequence involved in these interactions. Here, we describe how to perform ChIP-seq starting with whole tissues or cell lines, and ending with millions of short sequencing tags that can be aligned to the reference genome of the species under investigation. We also outline additional procedures to recover ChIP-chip libraries for ChIP-seq and discuss contemporary issues in data analysis. (C) 2009 Elsevier Inc. All rights reserved.
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