Journal
METHODS
Volume 47, Issue 1, Pages 25-36Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2008.10.010
Keywords
Abortive initiation; Promoter escape; Steady-state transcription reactions; Quantitative initiation parameters; Abortive RNA sequencing
Funding
- NSF [HRD-9252956, RUI-0077941]
- NIH [R15 GM55907]
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R15GM055907] Funding Source: NIH RePORTER
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Abortive initiation, when first discovered, was an enigmatic phenomenon, but fully three decades hence, it has been shown to be an integral step in the transcript initiation process intimately tied to the promoter escape reaction undergone by RNA polymerase at the initiation-elongation transition. A detailed understanding of abortive initiation-promoter escape has brought within reach a full description of the transcription initiation mechanism. This enormous progress was the result of convergent biochemical, genetic, and biophysical investigations propelled by parallel advances in quantitation technology. This chapter discusses the knowledge gained through the biochemical approach and a high resolution method that yields quantitative and qualitative information regarding abortive initiation-promoter escape at a promoter. (C) 2008 Elsevier Inc. All rights reserved
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