Journal
METHODS
Volume 44, Issue 3, Pages 222-228Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2007.11.007
Keywords
apoptosis; mitochondria; time-lapse confocal microscopy; cytochrome c; caspase
Funding
- NCI NIH HHS [P01 CA069381-120010, P01 CA069381] Funding Source: Medline
- NIAID NIH HHS [R01 AI047891-10, R01 AI044828-11, R01 AI040646, R01 AI047891, R01 AI047891-05, R01 AI040646-12A1, R01 AI044828] Funding Source: Medline
- NIGMS NIH HHS [R37 GM052735-17, R37 GM052735] Funding Source: Medline
Ask authors/readers for more resources
The use of live cell microscopy has made a number of contributions to the study of apoptosis. Many of the tools and techniques are available that allow us to image the key events that occur during cell death including mitochondrial outer membrane permeabilization, mitochondrial transmembrane potential changes, translocation of Bcl-2 family members, caspase activation, phosphatidylserine flip and plasma membrane rupture. We discuss these techniques here and highlight the advantages and drawbacks of using such approaches to study apoptosis. (C) 2007 Elsevier Inc. All rights reserved.
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