High-throughput, multi-platform metabolomics on very small volumes: 1H NMR metabolite identification in an unadulterated tube-in-tube system

As small animal models of disease become more widely used, there is increasing importance and potential for characterizing their metabolomes. However, as the animal becomes smaller, the amounts of biofluids such as urine and cerebral spinal fluid available for metabolomic studies are more limited. Further, in multi-platform systems biology when the same small sample must be used for several analyses, it is a frequent requirement that no additions are made to the sample (even as simple as D2O or an NMR chemical shift reference) to maintain sample integrity. Herein we describe a method for high-throughput H-1-NMR studies using similar to 30 A mu l volumes, suitable for biofluid matrices. The compartmentalization of the sample and NMR standards, however, requires chemical shift corrections due to bulk magnetic susceptibility and ionic strength changes for metabolite profiling using a reference library or data-binning of the chemical shift axis. This set-up minimizes the cost of individual data collection per small animal and is suitable for high-throughput, longitudinal, multimodal metabolomic studies of biofluids available in limited quantities.