Journal
METABOLOMICS
Volume 9, Issue 3, Pages 558-563Publisher
SPRINGER
DOI: 10.1007/s11306-012-0490-9
Keywords
NMR spectroscopy; Metabolomics; Compound screening; Automation; Data collection; Python scripting
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Funding
- National Center for Research Resources [5P41RR002301-27]
- National Institute of General Medical Sciences from the National Institutes of Health [8 P41 GM103399-27]
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To facilitate the high-throughput acquisition of nuclear magnetic resonance (NMR) experimental data on large sets of samples, we have developed a simple and straightforward automated methodology that capitalizes on recent advances in Bruker BioSpin NMR spectrometer hardware and software. Given the daunting challenge for non-NMR experts to collect quality spectra, our goal was to increase user accessibility, provide customized functionality, and improve the consistency and reliability of resultant data. This methodology, NMRbot, is encoded in a set of scripts written in the Python programming language accessible within the Bruker BioSpin TopSpin (TM) software. NMRbot improves automated data acquisition and offers novel tools for use in optimizing experimental parameters on the fly. This automated procedure has been successfully implemented for investigations in metabolomics, small-molecule library profiling, and protein-ligand titrations on four Bruker BioSpin NMR spectrometers at the National Magnetic Resonance Facility at Madison. The investigators reported benefits from ease of setup, improved spectral quality, convenient customizations, and overall time savings.
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