Journal
METABOLOMICS
Volume 10, Issue 3, Pages 508-523Publisher
SPRINGER
DOI: 10.1007/s11306-013-0597-7
Keywords
Citrus; Bud mutation; Metabolomics; Direct analysis in real-time; LC-ESI; Mass spectrometry
Categories
Funding
- National Basic Research Program of China (973 Program) [2011CB100601]
- National Natural Science Foundation of China [31330066, 31201612]
- Ministry of Education of China [20120146120032]
- National Science Foundation [IOS1146589]
- Direct For Biological Sciences
- Division Of Integrative Organismal Systems [1146589] Funding Source: National Science Foundation
Ask authors/readers for more resources
The direct analysis in real-time (DART) ion source and HPLC-electrospray mass spectrometry were applied in non-targeted metabolomic analyses of fruits of an orange bud mutant, 'Hong Anliu' along with its parental wild-type, 'Anliu'. Fruits of the two isogenic cultivars were sampled at three different ripening stages, i.e. 120, 170 and 220 days after flowering. More than 130 metabolites were tentatively identified, including acids, sugars, flavonoids, alkaloids, limonoids, coumarins, amino acids, and plant hormones. Metabolomic analyses revealed that, compared to its wild type, the bud mutant fruit is characterized by higher levels of monosaccharides and disaccharides and lower levels of organic acids such as citric acid, malic acid and quinic acid, which agrees well with the anticipated fruit quality benefits of the mutation. In addition, many secondary metabolites, such as flavonoids, showed significant differences between the two genotypes, indicating that the whole fruit metabolome is significantly changed due to the bud mutation. This study provided a comprehensive assessment of metabolites in orange fruits, and revealed metabolomic differences in fruits between two isogenic orange genotypes. The results are helpful for understanding how the bud mutation in 'Hong Anliu' impacts the physiological and biochemical processes of orange fruits.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available