Journal
METABOLOMICS
Volume 8, Issue 4, Pages 634-642Publisher
SPRINGER
DOI: 10.1007/s11306-011-0355-7
Keywords
Ricin; Ricinus communis; Metabolomics; Provenance; Cultivar
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Funding
- National Security Science and Technology (NSST) branch of the Department of Prime Minister and Cabinet, Emergency Management Australia
- Australian Federal Police through NSST
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Eight specimens of six known cultivars of Ricinus communis were investigated for differences in their metabolome that could be used to determine both provenance and cultivar. Seven replicates of three seeds per specimen were subjected to H-1 NMR analysis, with the collected data further investigated using multivariate statistical analysis (OPLS-DA), resulting in class separations according to provenance. Analysis of loadings plots in addition to further chemical analysis of the extracts allowed for the identification of phenylalanine, ricinine, the N-demethyl and O-demethyl analogues of ricinine, and sucrose as important molecular markers for particular cultivars. To test the strength of the model, extracts generated from blinded specimens were used as a prediction set and were correctly classified according to provenance and cultivar.
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