Journal
METABOLISM-CLINICAL AND EXPERIMENTAL
Volume 58, Issue 3, Pages 361-367Publisher
W B SAUNDERS CO-ELSEVIER INC
DOI: 10.1016/j.metabol.2008.10.009
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In overweight patients (OW), the increased peripheral activity of the endocannabinoid system in visceral adipose tissue (VAT) may be mediated by cannabinoid type I (CB I) receptor expression. We determined whether CB I receptor splice variants and messenger RNA (mRNA) levels in perirenal and Subcutaneous adipose tissues are associated with obesity and metabolic syndrome (MetS). Gene expression with multiple-primers real-time polymerase chain reaction (TaqMan; Applied Biosystem, Weiterstadt, Germany) was performed to study VAT and paired Subcutaneous adipose tissue (SAT) mRNA from 36 consecutive patients undergoing nephrectomy. Cannabinoid type I A and (CBIE mRNAs variants with the longer version of exon 4 were expressed. The CB I expression in perirenal VAT significantly correlated with body mass index (BMI). Paired subcutaneous/perirenal samples from normal-weight patients (BMI <25 kg/m(2)) showed higher CB I expression in SAT (P = .002), whereas in OW (BMI >= 25 kg/m(2)), the higher CBI expression was in VAT (P = .038). In unpaired samples, SAT of normal-weight patients had significantly higher CB1 mRNA levels compared with SAT of OW, whereas higher CB I expression (P = .009) was found in VAT of OW (n = 25). Overweight patients with increased visceral CB I expression had higher waist circumference (P < .01), insulin (P < .01), and homeostasis model assessment index (P < .01). In addition, patients with the MetS (n = 22) showed higher CB1 expression in perirenal adipose tissues (P = .007). Visceral adipose CB I expression correlated with BMI. Overweight patients and those with MetS showed a CB1 expression pattern Supporting a CBI-mediated overactivity of the endocannabinoid system in human VAT. (C) 2009 Elsevier Inc. All rights reserved.
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