Journal
METABOLIC ENGINEERING
Volume 21, Issue -, Pages 2-8Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymben.2013.10.011
Keywords
Escherichia coli; Flavonoid; Naringenin; Metabolite sensor; Whole-cell biotransformation; Quercetin
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Funding
- Novo Nordisk Foundation
- Novo Nordisk Fonden [NNF10CC1016517] Funding Source: researchfish
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This study describes the construction of two flavonoid biosensors, which can be applied for metabolic engineering of Escherichiu coli strains. The biosensors are based on transcriptional regulators combined with autofluorescent proteins. The transcriptional activator FcleR from Ilerbaspirillum seropedicae SmR1 responds to naringenin, while the repressor QdoR from Bacillus subtilis is inactivated by quercetin and kaempferol. Both biosensors showed over a 7-fold increase of the fluorescent signal after addition of their specific effectors, and a linear correlation between the fluorescence intensity and externally added flavonoid concentration. The QdoR-biosensor was successfully applied for detection of kaempferol production in vivo at the single cell level by fluorescence-activated cell sorting. Furthermore, the amount of kaemplerol produced highly correlated with the specific fluorescence of E. colt cells containing a flavonol synthase from Arabidopsis thaliana (fls1). We expect the designed biosensors to be applied for isolation of genes involved in flavonoid biosynthetic pathways. (C) 2013 The Authors. Published by Elsevier Inc. on behalf of International Metabolic Engineering Society. All rights reserved.
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