Journal
METABOLIC ENGINEERING
Volume 15, Issue -, Pages 174-183Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymben.2012.07.010
Keywords
Isoprenoids; S. cerevisiae; Carotenoids; Biofuel; Bisabolene
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Funding
- U. S. Department of Energy, Office of Science, Office of Biological and Environmental Research [DE-AC02-05CH11231]
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Beside their essential cellular functions, isoprenoids have value as pharmaceuticals, nutriceuticals, pesticides, and fuel alternatives. Engineering microorganisms for production of isoprenoids is relatively easy, sustainable, and cost effective in comparison to chemical synthesis or extraction from natural producers. We introduced genes encoding carotenoid biosynthetic enzymes into the haploid yeast deletion collection to identify gene deletions that improved isoprenoid production. Deletions that showed significant improvement in carotenoid production were further screened for production of bisabolene, an isoprenoid alternative to petroleum-derived diesel. Combining those deletions with other mevalonate pathway modifications increased production of bisabolene from 40 mg/L to 800 mg/L in shake-flask cultures. In a fermentation process, this engineered strain produced 5.2 g/L of bisabolene. (C) 2012 Published by Elsevier Inc.
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