Journal
METABOLIC ENGINEERING
Volume 14, Issue 5, Pages 487-495Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymben.2012.07.004
Keywords
Evolutionary engineering; Recombination; Clonal interference; Complex phenotypes
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Funding
- National Science Foundation GRF program [NSF MCB-1054276]
- Texas Engineering Experimental Station
- Direct For Biological Sciences
- Div Of Molecular and Cellular Bioscience [1054276] Funding Source: National Science Foundation
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Evolutionary engineering typically involves asexual propagation of a strain to improve a desired phenotype. However, asexual populations suffer from extensive clonal interference, a phenomenon where distinct lineages of beneficial clones compete and are often lost from the population given sufficient time. Improved adaptive mutants can likely be generated by genetic exchange between lineages, thereby reducing clonal interference. We present a system that allows continuous in situ recombination by using an Esherichia coli F-based conjugation system lacking surface exclusion. Evolution experiments revealed that Hfr-mediated recombination significantly speeds adaptation in certain circumstances. These results show that our system is stable, effective, and suitable for use in evolutionary engineering applications. (C) 2012 Elsevier Inc. All rights reserved.
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