Journal
METABOLIC ENGINEERING
Volume 13, Issue 6, Pages 656-665Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymben.2011.08.002
Keywords
Metabolic flux analysis; Cyanobacteria; Isotopomer analysis; Mass spectrometry; Photoautotrophic metabolism; Carbon fixation
Categories
Funding
- NSF [BES 0348458]
- NIH [R01 DK075850, F32 DK072856]
- Emerging Frontiers
- Direct For Biological Sciences [1219603] Funding Source: National Science Foundation
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Understanding in vivo regulation of photoautotrophic metabolism is important for identifying strategies to improve photosynthetic efficiency or re-route carbon fluxes to desirable end products. We have developed an approach to reconstruct comprehensive flux maps of photoautotrophic metabolism by computational analysis of dynamic isotope labeling measurements and have applied it to determine metabolic pathway fluxes in the cyanobacterium Synechocystis sp. PCC6803. Comparison to a theoretically predicted flux map revealed inefficiencies in photosynthesis due to oxidative pentose phosphate pathway and malic enzyme activity, despite negligible photorespiration. This approach has potential to fill important gaps in our understanding of how carbon and energy flows are systemically regulated in cyanobacteria, plants, and algae. (C) 2011 Elsevier Inc. All rights reserved.
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