Journal
METABOLIC ENGINEERING
Volume 13, Issue 2, Pages 194-203Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymben.2010.12.005
Keywords
Targeted proteomics; Selected-Reaction Monitoring (SRM); Mevalonate pathway; Amorphadiene production; Metabolic pathway optimization; E. coli
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Funding
- U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research [DE-AC02-05CH11231]
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Successful metabolic engineering relies on methodologies that aid assembly and optimization of novel pathways in microbes. Many different factors may contribute to pathway performance, and problems due to mRNA abundance, protein abundance, or enzymatic activity may not be evident by monitoring product titers. To this end, synthetic biologists and metabolic engineers utilize a variety of analytical methods to identify the parts of the pathway that limit production. In this study, targeted proteomics, via selected-reaction monitoring (SRM) mass spectrometry, was used to measure protein levels in Escherichia coli strains engineered to produce these squiterpene, amorpha-4,11-diene. From this analysis, two mevalonate pathway proteins, mevalonate kinase (MK) and phosphomevalonate kinase (PMK) from Saccharomyces cerevisiae, were identified as potential bottlenecks. Codon-optimization of the genes encoding MK and PMK and expression from a stronger promoterled to significantly improved MK and PMK protein levels and over three-fold improved final amorpha-4,11-diene titer(> 500 mg/L). (C) 2011 Elsevier Inc. All rights reserved.
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