Journal
METABOLIC ENGINEERING
Volume 13, Issue 5, Pages 455-463Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymben.2011.04.005
Keywords
Metabolic engineering; Tyrosine ammonia lyase; Resveratrol biosynthesis; Codon usage; Low-affinity high-capacity transporter (araE)
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Funding
- DOE [DE-SC0001295]
- NSF [MCB-0923779]
- USDA [2010-65116-20514]
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [0923779] Funding Source: National Science Foundation
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Resveratrol is a unique, natural polyphenolic compound with diverse health benefits. In the present study, we attempted to improve resveratrol biosynthesis in yeast by different methods of metabolic engineering. We first mutated and then re-synthesized tyrosine ammonia lyase (TAL) by replacing the bacteria codons with yeast-preferred codons, which increased translation and improved p-coumaric acid and resveratrol biosynthesis drastically. We then demonstrated that low-affinity, high-capacity bacterial araE transporter could enhance resveratrol accumulation, without transporting resveratrol directly. Yeast cells carrying the araE gene produced up to 2.44-fold higher resveratrol than control cells. For commercial applications, resveratrol biosynthesis was detected in sucrose medium and fresh grape juice using our engineered yeast cells. In collaboration with the Chaumette Winery of Missouri, we were able to produce resveratrol-containing white wines, with levels comparable to the resveratrol levels found in most red wines. (C) 2011 Elsevier Inc. All rights reserved.
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