Journal
METABOLIC ENGINEERING
Volume 12, Issue 3, Pages 233-240Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymben.2009.12.001
Keywords
Micromonas pusilla; Delta 6-desaturase; Acyl-CoA; SDA; LC-PUFA; Omega-3
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Long-chain (>= C-20) polyunsaturated fatty acids (LC-PUFA) EPA and DHA (20:5(Delta 5,8,11,14,17) and 22:6(Delta 4,7,10,13,16,19)) have well-documented health benefits against coronary heart disease, rheumatoid arthritis and other disorders. Currently, the predominant sources of these fatty acids are marine fish and algal oils, but research is being conducted to ensure that a sustainable, land-based production system can be developed. We here describe the metabolic engineering of an artificial pathway that produces 26% EPA in leaf triacylglycerol using a newly-identified Delta 6-desaturase from the marine microalga Micromonas pusilla. We also demonstrate that this enzyme appears to function as an acyl-CoA desaturase that has preference for omega 3 substrates both in planta and in yeast. Phylogenetic analysis indicates that this desaturase shares highly conserved motifs with previously described acyl-CoA Delta 6-desaturases. Crown Copyright (C) 2009 Published by Elsevier Inc. All rights reserved.
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