Journal
MENOPAUSE-THE JOURNAL OF THE NORTH AMERICAN MENOPAUSE SOCIETY
Volume 17, Issue 6, Pages 1174-1181Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/gme.0b013e3181e39a2b
Keywords
1,25-Hydroxyvitamin D-3; 25-Hydroxyvitamin D 1-alpha hydroxylase; Fructus Ligustri Lucidi; Vitamin D receptor; Vitamin D metabolism
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Funding
- Niche Area Research Scheme [1-BB8N]
- Research Committee of The Hong Kong Polytechnic University
- State Key Laboratory of Chinese Medicine and Molecular Pharmacology, Shenzhen
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Objective: The present study was designed to determine whether Fructus Ligustri Lucidi (FLL) ethanol extract can directly regulate vitamin D metabolism both in vivo and in vitro. Methods: Eleven-month-old, aged Sprague-Dawley sham-operated and ovariectomized (OVX) female rats were fed a normal-calcium (Ca) diet (0.6% Ca, 0.65% phosphorus) and received either FLL (700 mg/kg) or vehicle daily for 12 weeks. The in vitro effects of FLL on vitamin D metabolism were studied using primary cultures of the rat renal proximal tubules. mRNA and protein expressions of 25-hydroxyvitamin D-1 alpha hydroxylase (1-OHase) and vitamin D receptor (VDR) in the kidney and proximal tubule were measured using real-time polymerase chain reaction and Western blotting, respectively. The concentrations of 1,25-dihydroxyvitamin D-3 (1,25(OH)(2)D-3) synthesized by renal 1-OHase were measured by a competitive enzyme immunoassay. Results: FLL treatment significantly increased serum 1,25(OH)(2)D-3 levels in both sham (P < 0.01) and OVX (P < 0.05) rats. FLL increased renal 1-OHase and VDR protein and mRNA expressions in sham rats. Protein expression of renal 1-OHase, but not VDR, was also up-regulated in OVX rats during FLL treatment. 1-OHase mRNA and 1-OHase activity were increased by FLL treatment in primary cultures of renal proximal tubule cells. Conclusions: FLL could increase the circulating levels of 1,25(OH)(2)D-3 in vivo in aged female rats by directly stimulating 1-OHase activity. Thus, it might be an ideal oral agent that can help to improve the ability to induce 1,25(OH)(2)D-3 synthesis and Ca balance in postmenopausal women who are of high risk of developing osteoporosis.
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