Journal
MELANOMA RESEARCH
Volume 21, Issue 3, Pages 253-256Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/CMR.0b013e328345333b
Keywords
Akt3; apoptosis; melanoma cell line; microRNA; miR-125b; proliferation; senescence
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Funding
- Research Foundation of Rigshospitalet
- Aage Bangs Foundation
- Augustinus Foundation
- Axel Muusfeldts Foundation
- Else and Mogens Wedell-Wedellsborgs Foundation
- Danish Cancer Society
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MicroRNAs (miRNAs) are small noncoding RNA molecules involved in gene regulation. Aberrant expression of miRNA has been associated with the development or progression of several diseases, including cancer. In a previous study, we found that the expression of miRNA-125b (miR-125b) was two-fold lower in malignant melanoma producing lymph node micrometastases than in nonmetastasizing tumors. To get further insight into the functional role of miR-125b, we assessed whether its overexpression or silencing affects apoptosis, proliferation, or senescence in melanoma cell lines. We showed that overexpression of miR-125b induced typical senescent cell morphology, including increased cytoplasmatic/nucleus ratio and intensive cytoplasmatic beta-galactosidase expression. In contrast, inhibition of miR-125b resulted in 30-35% decreased levels of spontaneous apoptosis. We propose that downregulation of miR-125b in an early cutaneous malignant melanoma can contribute to the increased metastatic capability of this tumor. Melanoma Res 21:253-256 (C) 2011 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.
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