Journal
MELANOMA RESEARCH
Volume 18, Issue 2, Pages 95-103Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/CMR.0b013e3282f628df
Keywords
animal model; hepatic metastasis; micrometastasis; uveal melanoma
Categories
Funding
- NCI NIH HHS [R01 CA126447-07, R01 CA126447-06, R01 CA126447] Funding Source: Medline
- NEI NIH HHS [R24 EY017045, P30 EY006360-23] Funding Source: Medline
Ask authors/readers for more resources
The purpose of the study is to develop a mouse ocular melanoma model with human uveal melanoma cells that forms hepatic micrometastases. Human uveal melanoma Mel290 cells were transfected with a lentiviral-enhanced green fluorescent protein (EGFP) expression vector. Proliferation assays were performed by comparing Mel290-EGFP and Mel290 cells. After stable expression of EGFP and proliferation was ascertained, 1 x 10(6) Mel290-EGFP cells were introduced into NU/NU mice by posterior compartment (PC) inoculation or tall vein injection. Control groups were inoculated or injected with Me1290 cells. Ocular and hepatic frozen sections were examined by fluorescence microscopy, and the number of hepatic micrometastases was determined. EGFP expression was observed at 24 h after transfection. At 72 h after transfection, more than 70% of Mel290 cells expressed EGFR At 45 days (six passages), 90% of Me1290 cells stably expressed EGFR Histologic examination showed that Mel290-EGFP cells formed hepatic micrometastases after either PC inoculation or tail vein injection. A significant difference in the number of hepatic micrometastases between PC inoculation and tail vein injection (P<0.01) was observed. Mel290-EGFP cells stably expressed green fluorescent protein in vitro at 45 days (six passages). These cells formed hepatic micrometastases in NU/NU mice after PC inoculation or tail vein injection, with significantly more micrometastases developing in the PC inoculation model than after tail vein injection.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available