Synthesis, preliminary cytotoxicity evaluation of new 3-formylchromone hydrazones and phosphorohydrazone derivatives of coumarin and chromone

Reactions of chromone derivatives 1-4 with N-substituted hydrazines were described. Hydrazones (6, 7a, 8a-c, 9b-e, 10e, 11e, 12) were evaluated for cytotoxicity (MTT test) against HL-60 and NALM-6 leukemia cells. Phosphorohydrazone of 3-formylchromone 8a and hydrazone of 2-amino-3-chromone derivative 11e showed appreciable cytotoxicity. The cytotoxicity indices of 8a, 11e, and 12 were higher on drug-resistant HL-60 ADR cells in comparison to HL-60. Compounds 8a and 11e were tested for their ability to induce cytochrome c translocation from mitochondria to cytosol.