Journal
MEDICINAL CHEMISTRY
Volume 8, Issue 3, Pages 474-480Publisher
BENTHAM SCIENCE PUBL LTD
DOI: 10.2174/1573406411208030474
Keywords
Antibacterial activity; drug screening; antibiotic-phytochemicals synergy; isothiocyanates; pathogenic bacteria
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Funding
- Portuguese Foundation for Science and Technology [SFRH/BGCT/33354/2008, SFRH/BPD/65029/2009]
- Fundação para a Ciência e a Tecnologia [SFRH/BPD/65029/2009, SFRH/BGCT/33354/2008] Funding Source: FCT
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The emergence of new diseases and the resurgence of several infections that were controlled in the past, associated with recent increase of bacterial resistance have created the necessity for more studies towards the development of new antimicrobials and new treatment strategies. The aim of the present study was to evaluate the in vitro synergy between different classes of important glucosinolates hydrolysis products-isothiocyanates with antibiotics (gentamicin and vancomicin), against important pathogenic bacteria: Escherichia coli, Enterococcus faecalis, Listeria monocytogenes, Pseudomonas aeruginosa and Staphylococcus aureus. A disc diffusion method was used to evaluate the antibacterial activity. The antimicrobial activity of phytochemicals and combinations between gentamicin, vancomicin and phytochemicals were quantitatively assessed by measuring the inhibitory halos. The results showed a selective antimicrobial effect of isothiocyanates, and this effect was strictly related with their chemical structure. In general the benzylisothiocyanate was the most effective compound against both Gram-positive and Gram-negative bacteria. The Listeria monocytogenes and Staphylococcus aureus were the bacteria, most affected either by the phytochemicals alone or by the combination phytochemical-antibiotic. The bacteria Pseudomonas aeruginosa was the less affected pathogen. The most important synergism detected occurred between the commercial antibiotics with benzylisothiocyanate and 2-phenylethylisothiocyanate. In conclusion, some isothiocyanates are effective inhibitors of in vitro bacterial growth, and they can act synergistically with antibiotics.
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