Journal
MEDICAL SCIENCE MONITOR
Volume 17, Issue 10, Pages MT91-MT96Publisher
INT SCIENTIFIC INFORMATION, INC
DOI: 10.12659/MSM.881967
Keywords
Escherichia coli; capillary zone electrophoresis; infection
Categories
Funding
- Ministry of Science and Higher Education [3694/B/H03/2011/40]
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Background: Escherichia coli is a Gram-negative bacterium which is a basic, symbiotic element of the physiological flora of the large intestine of humans and warm-blooded animals. However, in specific cases it may become a very dangerous pathogen (eg, diarrhoea, infection of the urinary tract, lungs, and generalized infections). Its early detection, as a cause of infectious disease, helps to achieve optimal treatment results; however, classical microbiological tests require at least 24 hours from sample taking to diagnosis. Material/Methods: We present a unique solution based on CZE technologies enabling identification of E. coli presence in studied sample within half an hour. Altogether, 30 E. coli-infected wounds and ulcerations were examined, comparing the results obtained by classical culture method with the result of capillary zone electrophoresis (CZE) electropherogram. Results: The method, which does not require any preparation of the sample, achieved 86.7% sensitivity and 85% specificity in the examined clinical material (infections of surgical wounds). Conclusions: The obtained results enable reliable, very fast testing for E. coli as a pathogen.
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