Journal
RNA BIOLOGY
Volume 12, Issue 3, Pages 238-247Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/15476286.2015.1017238
Keywords
chinese hamster ovary; microRNA; pathway regulation; redundancy; screen
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Funding
- Postgraduate Scholarships Act of the Ministry for Science, Research and Arts of the federal state government of Baden-Wurttemberg, Germany
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Understanding the multifaceted nature of microRNA (miRNA) function in mammalian cells is still a challenge. Commonly accepted principles of cooperativity and multiplicity of miRNA function imply that individual mRNAs can be targeted by several miRNAs whereas a single miRNA may concomitantly regulate a subset of different genes. However, there is a paucity of information whether multiple miRNAs regulate critical cellular events and thereby acting redundantly. To gain insight into this notion, we conducted an unbiased high-content miRNA screen by individually introducing 1139 miRNA mimics into Chinese hamster ovary (CHO) cells. We discovered that 66% of all miRNAs significantly impacted on proliferation, protein expression, apoptosis and necrosis. In summary, we provide evidence for a substantial degree of redundancy among miRNAs to maintain cellular homeostasis.
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