Journal
MEDICAL PHYSICS
Volume 36, Issue 6, Pages 2320-2323Publisher
AMER ASSOC PHYSICISTS MEDICINE AMER INST PHYSICS
DOI: 10.1118/1.3137572
Keywords
biochemistry; biomedical optical imaging; blood; blood vessels; ear; haemodynamics; laser applications in medicine; optical microscopy; oxygen; photoacoustic effect; surgery
Funding
- National Institutes of Health [R01 EB000712, R01 NS46214, R01 EB008085, U54 CA136398]
- Endra, Inc.
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Optical-resolution photoacoustic microscopy (OR-PAM) has been validated as a valuable tool for label-free volumetric microvascular imaging. More importantly, the advantages of noninvasiveness and measurement consistency suggest the use of OR-PAM for chronic imaging of intact microcirculation. Here, such chronic imaging is demonstrated for the first time by monitoring the healing process of laser-induced microvascular lesions in a small animal model in vivo. The central part of a 1 mm by 1 mm region in a nude mouse ear was treated under a continuous-wave laser to create a microvascular lesion for chronic study. The region of interest was imaged before the laser treatment, immediately after the treatment, and throughout the healing process using both the authors' OR-PAM system and a commercial transmission-mode optical microscope. Three-dimensional microvascular morphology and blood oxygenation information were imaged simultaneously at capillary-level resolution. Transmission-mode optical microscopic images were acquired for comparison. OR-PAM has potential important applications in microcirculatory physiology or pathophysiology, tumor angiogenesis, laser microsurgery, and neuroscience.
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