Journal
MEDICAL ONCOLOGY
Volume 28, Issue -, Pages S433-S438Publisher
HUMANA PRESS INC
DOI: 10.1007/s12032-010-9660-7
Keywords
Extracellular signal-regulated kinase; Glioma; Matrix metalloproteinase-7; Matrix metalloproteinase-14; Extracellular matrix
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Funding
- Natural Science Foundation of China [30800451, 30872656, 30700861, 30670723, 30973079]
- Scientific and Technological Research Projects in Colleges and Universities of Liaoning Province [2008850]
- special fund for Scientific Research of Doctor-degree Subjects in Colleges and Universities [20092104110015]
- Scientific and Technological Planning Projects of Shenyang [1072033-1-00, 1081266-9-00]
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The purpose of this study is to determine whether the expressions of MMP-7 and MMP-14 are associated with the ERK 1/2 signaling pathway in human brain gliomas of different pathological grades. Immunohistochemistry and western blot methods were used to determine the expressions of MMP-7, MMP-14 and the phosphorylation status of ERK1/2 in 73 cases of human brain glioma specimens and two cases of normal brain tissues. Results indicated that the protein expression levels of MMP-7, MMP-14 and ERK1/2 phosphorylation level were all elevated with the increasing pathological grades in brain glioma tissues, and correlation assay indicated that the level of ERK1/2 phosphorylation was positively correlated with protein expression levels of MMP-7 and MMP-14 in gliomas of different pathological grades respectively. Moreover, the impact of ERK1/2 inhibitor U0126 on the expressions of MMP-7 and MMP-14 was examined in human U87 glioma cells by western blot analysis. The expressions of MMP-7 and MMP-14 were significantly decreased in human U87 glioma cells after treatment with ERK1/2 inhibitor U0126. The above results suggest that the expressions of MMP-7 and MMP-14 may be associated with activation of ERK1/2 signaling pathway in human brain gliomas of different pathological grades.
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