Journal
MEDICAL MYCOLOGY
Volume 48, Issue 8, Pages 1049-1055Publisher
OXFORD UNIV PRESS
DOI: 10.3109/13693786.2010.496119
Keywords
Aspergillus fumigatus; airway epithelium; porcine tracheal epithelial cells; mRNA quantification
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Funding
- AFS-SET [EST-2007-63, EST-2008-27]
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Since the airway epithelium is the first tissue encountered by airborne fungal spores, specific models are needed to study this interaction. We developed such a model using primary porcine tracheal epithelial cells (PTEC) as a possible alternative to the use of primary human cells. PTEC were obtained from pigs and were cultivated in an air-liquid interface. Fluorescent brightener was employed to quantify the internalization of Aspergillus fumigatus conidia. Potential differences (Vt) and transepithelial resistances (Rt) after challenge with the mycotoxin, verruculogen, were studied. Primers for porcine inflammatory mediator genes IL-8, TNF-alpha, and GM-CSF were designed for a quantitative real-time PCR procedure to study cellular responses to challenges with A. fumigatus conidia. TEM showed the differentiation of ciliated cells and the PTEC ability to internalize conidia. The internalization rate was 21.9 +/- 1.4% after 8 h of incubation. Verruculogen (10
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