Journal
MEDIATORS OF INFLAMMATION
Volume 2013, Issue -, Pages -Publisher
HINDAWI LTD
DOI: 10.1155/2013/390890
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Funding
- China Scholarship Council
- Gansu Province Natural Science Foundation of China [0710RJZA037, 1208RJZA231]
- American Heart Association [SDG 0835169N, 12GRNT 11620029]
- American Diabetes Association Basic Sciences [1-11-BS-92]
- Major International (Regional) Joint Research Project [2008DFA31140, 2010DFA32660]
- Guangdong Natural Science Fund [10251008002000002, S2011010005836]
- Chinese Science and Technology Support Program [2011BAI11B22]
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Isoliquiritigenin (ISL), a simple chalcone-type flavonoid, is derived from licorice compounds and is mainly present in foods, beverages, and tobacco. Reactive oxygen species (ROS) is a critical factor involved in modulating cardiac stress response signaling during ischemia and reperfusion. We hypothesize that ISL as a natural antioxidant may protect heart against ischemic injury via modulating cellular redox status and regulating cardioprotective signaling pathways. The fluorescent probe H(2)DCFDA was used to measure the level of intracellular ROS. The glucose uptake was determined by 2-deoxy-D-glucose-H-3 accumulation. The IonOptix System measured the contractile function of isolated cardiomyocytes. The results demonstrated that ISL treatment markedly ameliorated cardiomyocytes contractile dysfunction caused by hypoxia. ISL significantly stimulated cardioprotective signaling, AMP-activated protein kinase (AMPK), and extracellular signal-regulated kinase (ERK) signaling pathways. The ROS fluorescent probe H(2)DCFDA determination indicated that ISL significantly reduced cardiac ROS level during hypoxia/ reoxygenation. Moreover, ISL reduced the mitochondrial potential (Delta psi) of isolated mouse cardiomyocytes. Taken together, ISL as a natural antioxidant demonstrated the cardioprotection against ischemic injury that may attribute to the activation of AMPK and ERK signaling pathways and balance of cellular redox status.
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