Journal
MECHANISMS OF DEVELOPMENT
Volume 130, Issue 1, Pages 54-60Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mod.2012.05.008
Keywords
Heat shock; Transcription factor; Root development; Endodermis; Lateral root cap; Stem cells
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Funding
- Deutsche Forschungsgemeinschaft [SFB446]
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The functions of plant class B-heat shock factors (Hsfs) are not well understood. Hsfs belonging to this group differ from class A-Hsfs in structural features of the oligomerization domain and by the absence of a typical AHA motif for transcriptional activation. AtHsfB4 is expressed in different parts of the plants with highest levels in root tissue. Transgenic Arabidopsis plants overexpressing (OE) HsfB4 by CaMV-35S-promoter showed massively enhanced levels of Hsf mRNAs. The root surface of OE-plants was rough and cells became detached. Crossings with cell type specific root marker lines and confocal laser scanning microscopy provided clear evidence for a duplication of cells in the ground tissue and ectopic layers of lateral root cap (LRC) cells in HsfB4-OE plants. A duplication of endodermis cells occurs already during embryonic development, while the ectopic LRC cells are only detected during postembryonic growth. The mutant phenotypes of Hsf-OE plants are without precedence and indicate that class B-Hsfs may play an important role in root development. (c) 2012 Elsevier Ireland Ltd. All rights reserved.
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