Journal
MECHANISMS OF DEVELOPMENT
Volume 126, Issue 10, Pages 852-862Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mod.2009.07.006
Keywords
Myogenesis; Cardiogenesis; Chicken embryo; Somite; Myotome; Klhl31; Kelch-like; Wnt signaling; Shh signaling; Myf-5
Categories
Funding
- Arab Republic of Egypt, O.C
- BBSRC DTA studentship
- BBSRC [016444]
- MRC [600757]
- EU FP6 MYORES network of excellence
- MRC [G0600757] Funding Source: UKRI
- Medical Research Council [G0600757] Funding Source: researchfish
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Klhl31 is an orthologue of Drosophila Kelch and belongs to a family of Kelch-like proteins in vertebrates. Members of this family contain multiple protein domains, including an aminoterminal broad complex/tram-track/bric-a-brac (BTB) or poxvirus and zinc finger (POZ) domain, carboxy-terminal Kelch repeats and a central linker region. We show that Klhl31 is highly expressed in the developing heart, the somite myotome and later in differentiated skeletal muscle and the myocardium. In developing somites expression of Klhl31 was initiated in the epaxial domain of the myotome, shortly after the skeletal muscle specific bHLH transcription factor, MyoD, was first expressed. Klhl31 remained expressed in skeletal muscle throughout embryonic and fetal development. Tissue ablations and rescue experiments that regulate myogenesis also govern expression of Klhl31 expression in somites. In particular, axial tissues, neural tube, floor plate and notochord, and surface ectoderm, provide combinatorial cues for myogenesis and the appropriate expression of Klhl31. We show that a combination of myogenic signals, Shh and either Wnt-1 or Wnt-6, are sufficient for Klhl31 expression in the dorsal somite. Furthermore, ectopic expression of Myf-5 led to expression of Klhl31 in the developing neural tube, indicating that Klhl31 is a novel and integral part of vertebrate myogenesis. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
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