Journal
MECHANISMS OF AGEING AND DEVELOPMENT
Volume 131, Issue 2, Pages 133-143Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.mad.2009.12.011
Keywords
Aging; Clark-type electrode; Respiration; Cortex; Mitochondria; Oxidative damage
Categories
Funding
- NIH [AG21981]
- NIH-NIDA [1T32 DA 022738-01]
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This study probed possible age-related changes in mitochondrial bioenergetics in naive Fischer 344 rats. Synaptic and extrasynaptic mitochondria were isolated from the cortex of one hemisphere of young (3-5 months), middle (12-14 months), or aged (22-24 months) rats. Respiration parameters were obtained using a Clarke-type electrode. Aged rats displayed no significant alterations in respiration, indicating mitochondria must be more resilient to the aging process than previously thought. Synaptic mitochondria displayed lower respiration capacities than the extrasynaptic fraction. Aged F344 rats appear capable of normal electron transport chain function without declines in ability to produce ATP. Markers of cortical oxidative damage (3-nitrotyrosine [3-NT],4-hydroxynonenal [4-HNE], and protein carbonyls [PC]) were collected from the post-mitochondrial supernatant (PMS) from the contralateral hemisphere, and from mitochondrial samples following respiration analysis. Age-related increases in PC and 3-NT levels were found in synaptic mitochondria, whereas significant extrasynaptic elevations were only found in middle aged rats. These findings support an age-related increase in oxidative damage in the cortex, while proposing the two fractions of mitochondria are differentially affected by the aging process. Levels of oxidative damage that accumulates in the cortex with age does not appear to significantly impair cortical mitochondrial respiration of F344 rats. (C) 2010 Elsevier Ireland Ltd. All rights reserved.
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