Journal
MEAT SCIENCE
Volume 98, Issue 1, Pages 41-46Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.meatsci.2014.04.002
Keywords
Soybean detection; Adulteration; Food allergen; Meat products; Real-time PCR
Categories
Funding
- Fundacao para a Ciencia e a Tecnologia (FCT) [PEst-C/EQB/LA0006/2013]
- University of Porto Projectos Pluridisciplinares [IJUP2011-149]
- POPH - QREN [SFRH/BD/75091/2010]
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In the present work, we propose a normalised real-time quantitative PCR assay to determine the addition of soybean to meat products. The method proved to be a powerful tool for the quantification of soybean protein (dry basis) in the range of 0.01% to 6%, being successfully in-house validated. Its application was effective in the analysis of several meat products, indicating 2% of non-compliance with the food allergen labelling legislation, and some inconsistencies when comparing the declared with estimated amounts of soybean. This work highlights the importance of efficient tools to assess labelling statements of meat products, avoiding fraudulent practices. (C) 2014 Elsevier Ltd. All rights reserved.
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