Journal
MEAT SCIENCE
Volume 86, Issue 3, Pages 738-741Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.meatsci.2010.06.014
Keywords
Pyruvate; Mitochondria; Myoglobin; MRA; Oxygen consumption
Categories
Funding
- Beef Checkoff
- National Cattlemen's Beef Association
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Pyruvate can regenerate NADH through a variety of biochemical processes. However, no meat science research has determined if NADH produced via pyruvate can be used for mitochondria-mediated metmyoglobin reduction. Thus, our objectives were to assess the effects of pyruvate on mitochondria isolated from bovine cardiac muscle: oxygen consumption and metmyoglobin reduction at pH 5.6 and 7.4, 25 degrees C in vitro. Both mitochondria and myoglobin were isolated from fresh bovine hearts (n = 5). Mitochondria were reacted with pyruvate (50 mM), succinate (positive control; 50 mM), and antimycin A (mitochondria] inhibitor; 0.01 mM) and oxygen consumption was measured using a Clark oxygen electrode. Mitochondria (3 mg/mL) and metmyoglobin (0.15 mM) were reacted with either pyruvate, succinate, or antimycin A for 3 h. Addition of succinate and pyruvate increased oxygen consumption and metmyoglobin reduction at pH 5.6 and 7.4 (succinate>pyruvate, P<0.05). Addition of a complex Ill inhibitor (antimycin A) decreased (P<0.05) oxygen consumption as well as metmyoglobin reduction associated with pyruvate and succinate. Results from the current study suggest that pyruvate can increase the ability of mitochondria to consume oxygen and reduce metmyoglobin. (C) 2010 The American Meat Science Association. Published by Elsevier Ltd. All rights reserved.
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