Partial replacement of nitrite by annatto as a colour additive in sausage

For decreasing the use of nitrite in sausage in industrial conditions, annatto (Bixa orellana L.) powder ( 1% norbixin) was used in two different formulations of sausage (with 55% and 70% of meat) as a replacement for 20%, 40%, 60%, 80% and 100% nitrite. Then the qualitative characteristics of the samples such as colour (L*, a* and b*), microbial contamination and sensory tests (flavour and odour) were compared with the control (without annatto and with 100% nitrite) after 2, 10, 20 and 30 days from production and under the refrigerated condition. The Statistical comparison showed that in both formulations of sausage, the sample containing 60% annatto was the best sample for its colour (for higher a* and lower b*): yet, this sample also did not show any significant differences from the control for microbial contamination and sensory properties. (C) 2008 Elsevier Ltd. All rights reserved.