Journal
MEAT SCIENCE
Volume 83, Issue 1, Pages 57-61Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.meatsci.2009.03.015
Keywords
Cyt b gene; Mitochondrial DNA; Halal authentication; Forensic science; Meat; PCR-restriction fragment length polymorphism (RFLP); Species identification
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Funding
- Malaysian Government
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A method utilizing PCR-restriction fragment length polymorphism (RFLP) in the mitochondrial genes was developed for beef (Bos taurus), pork (Sus scrofa), buffalo (Bubalus bubali), quail (Coturnix coturnix), chicken (Gallus gallus), goat (Capra hircus), rabbit (Oryctolagus cuniculus) species identification and Halal authentication. PCR products of 359-bp were successfully obtained from the cyt b gene of these six meats. Alu1, BsofI, Rsa1, Mse1, and BstU1 enzymes were identified as potential restriction endonucleases to differentiate the meats. The genetic differences within the cyt b gene among the meat were successfully confirmed by PCR-RFLP. A reliable typing scheme of species which revealed the genetic differences among the species was developed. (C) 2009 Elsevier Ltd. All rights reserved.
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