Journal
MEAT SCIENCE
Volume 83, Issue 3, Pages 555-562Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.meatsci.2009.07.004
Keywords
Salmonella; Real-time PCR; ssrA Gene; IAC; Fresh meat; Detection
Categories
Funding
- Food Institutional Research Measure (FIRM)
- Department of Agriculture, Fisheries and Food, Ireland
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In this study, a combined enrichment/real-time PCR method for the rapid detection of Salmonella on fresh meat carcasses, was designed, developed and validated in-house following requirements outlined in ISO 16140:2003. The method included an 18 h non-selective enrichment in buffered peptone water (BPW) and a 6 h selective enrichment in Rappaport Vasilliadis Soya (RVS) broth, based on the traditional culture method, ISO 6579:2002. The real-time PCR assay included an internal amplification control (IAC), was 100% specific and was sensitive to one cell equivalent. The alternative method was validated against the traditional culture method and relative accuracy of 94.9%, sensitivity of 94.7% and specificity of 100% were determined using 150 fresh meat carcass swabs. This alternative method had a detection limit of 1-10 CFU/100 cm(2) for fresh meat carcass swabs and was performed in 26 h. Following further inter-laboratory Studies, this alternative method could be suitable for implementation in testing laboratories for the analysis of carcass swabs. (C) 2009 Elsevier Ltd. All rights reserved.
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