Journal
MATURITAS
Volume 78, Issue 2, Pages 123-130Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.maturitas.2014.04.002
Keywords
GPER; Cardiovascular diseases; Endothelium; Vascular smooth muscle cells; Vasodilation; Smooth muscle
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Funding
- Centre of Gender Medicine at the Karolinska Institutet
- Swedish Heart and Lung Foundation
- Karolinska Institutet
- Swedish Society of Medicine
- MIUR Grant entitled Preterm birth: molecular, biochemical and biophysical markers of the feto-placental unit [prot. 20102CHST5]
- Stiftelsen Olle Engkvist Byggare
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G-protein-coupled estrogen receptors (GPERs) have been proposed to mediate estrogen-mediated vasodilation. The presence of GPER-dependent vasodilation in human resistance-sized arteries (HRAs) or its signal transduction pathways have not been investigated. HRAs in subcutaneous fat tissues (biopsies from postmenopausal women (PMW), age-matched men (M) and pregnant women (PGW)) were mounted for in vitro isometric force recording. Vasodilation induced by G-1 (selective GPER-agonist, 3 p,M) from HRAs pre-contracted with norepinephrine amounted to 40 +/- 5% in PMW, significantly larger than those obtained from M (20 +/- 5%) or PGW (20 +/- 5%). L-NAME (nitric oxide (NO) synthase inhibitor) abolished these relaxations in PGW, attenuated them in PMW and had no effect in M. Immunohistochemical analysis confirmed the presence of GPER in both smooth muscle and endothelial cells of HRA with maximum expression in PGW. In cultured human umbilical vein endothelial cells (HUVECs), G-1 increased NO-synthesis concentration-dependently through higher expressions of endothelial NO-synthase (eNOS) and through enhanced phosphorylation of eNOS on Ser(1177). In conclusion, GPER vasodilates human resistance arteries through various activating mechanisms of the eNOS-signaling pathway. (C) 2014 Elsevier Ireland Ltd. All rights reserved.
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