Journal
MATRIX BIOLOGY
Volume 36, Issue -, Pages 39-50Publisher
ELSEVIER
DOI: 10.1016/j.matbio.2014.03.008
Keywords
Elastin; Self-assembly; Phase separation; Colloid; Interface
Categories
Funding
- Heart and Stroke Foundation of Ontario [T6725]
- Natural Science and Engineering Research Council of Canada RGPIN [375738-09]
- Canadian Institutes of Health Research [MOP111145]
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Elastin self-assembles from monomers into polymer networks that display elasticity and resilience. The first major step in assembly is a liquid-liquid phase separation known as coacervation. This process represents a continuum of stages from initial phase separation to early growth of droplets by coalescence and later maturation leading to fiber formation. Assembly of tropoelastin-rich globules is on pathway for fiber formation in vivo. However, little is known about these intermediates beyond their size distribution. Here we investigate the contribution of sequence and structural motifs from full-length tropoelastin and a set of elastin-like polypeptides to the maturation of coacervate assemblies, observing their growth, stability and interaction behavior, and polypeptide alignment within matured globules. We conclude that maturation is driven by surface properties, leading to stabilization of the interface between the hydrophobic interior and aqueous solvent, potentially through structural motifs, and discuss implications for droplet interactions in fiber formation. (C) 2014 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license
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