Journal
REVIEW OF SCIENTIFIC INSTRUMENTS
Volume 86, Issue 1, Pages -Publisher
AMER INST PHYSICS
DOI: 10.1063/1.4905330
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Funding
- CASIO grant (Japan)
- Konica Minolta (Japan)
- Japan Society for the Promotion of Science (JSPS) [24300112, 26640132]
- Cancer Research UK
- Cancer Research UK [9787] Funding Source: researchfish
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We provide an evaluation for an electrically tunable lens (ETL), combined with a microscope system, from the viewpoint of tracking intracellular protein complexes. We measured the correlation between the quantitative axial focus shift and the control current for ETL, and determined the stabilization time for refocusing to evaluate the electrical focusing behaviour of our system. We also confirmed that the change of relative magnification by the lens and associated resolution does not influence the ability to find intracellular targets. By applying the ETL system to observe intracellular structures and protein complexes, we confirmed that this system can obtain 10 nm order z-stacks, within video rate, while maintaining the quality of images and that this system has sufficient optical performance to detect the molecules. (C) 2015 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 Unported License.
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