Titanium dioxide-cellulose hybrid nanocomposite and its glucose biosensor application

This paper investigates the fabrication of titanium dioxide (TiO2)-cellulose hybrid nanocomposite and its possibility for a conductometric glucose biosensor. TiO2 nanoparticles were blended with cellulose solution prepared by dissolving cotton pulp with lithium chloride/N,N-dimethylacetamide solvent to fabricate TiO2-cellulose hybrid nanocomposite. The enzyme, glucose oxidase (GOx) was immobilized into this hybrid nanocomposite by physical adsorption method. The successful immobilization of glucose oxidase into TiO2-cellulose hybrid nanocomposite via covalent bonding between TiO2 and GOx was confirmed by X-ray photoelectron analysis. The linear response of the glucose biosensor is obtained in the range of 1-10 mM. This study demonstrates that TiO2-cellulose hybrid nanocomposite can be a potential candidate for an inexpensive, flexible and disposable glucose biosensor. (C) 2012 Elsevier B.V. All rights reserved.