Journal
MATERIALS SCIENCE AND ENGINEERING B-ADVANCED FUNCTIONAL SOLID-STATE MATERIALS
Volume 177, Issue 4, Pages 388-393Publisher
ELSEVIER
DOI: 10.1016/j.mseb.2012.01.002
Keywords
Magnesium; Apoptosis; Caspase-1; Bcl-2; In vitro test
Funding
- National Basic Research Program of China (973 Program) [2012CB619102]
- Natural Science Foundation of China [30901422]
- 863 High-tech Plan of China [2009AA03Z424]
- Shanghai Jiao Tong University [YG2010MS46]
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In this study, intestinal epithelial cells (IEC)-6 were cultured in different concentration extracts of Mg-6Zn alloys for different time periods. To achieve a total of three concentrations (100%. 60% and 20% concentration), the extracts were serially diluted with Dulbecco's modified Eagle medium High Glucose to observe a dose-response relationship. We studied the indirect effects of Mg-6Zn alloys on IEC-6 cells apoptosis. The apoptosis of IEC-6 cells was measured using flow cytonnetry. And the apoptosis of IEC-6 cells was evaluated by investigating the expression of caspase-land Bcl-2 using real-time polymerase chain reaction (PCR) and Western blotting tests. It was found that the levels of apoptosis in IEC-6 cells cultured in 100% Mg-6Zn alloy extracts were significantly higher than those in 60% and 20% extracts; the 100% extract can down-regulate expression of Bcl-2 after culture. The in vitro results indicated that the conspicuous alkaline environment and excessive Mg concentration, even Zn concentration caused by rapid corrosion of Mg-6Zn alloys promote IEC-6 cells apoptosis, although further experiments will be necessary to formally prove our conclusions. Therefore, the adjustment of the degradation rate is needed for using Mg-Zn alloy as a surgical suture material. (C) 2012 Elsevier B.V. All rights reserved.
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