Journal
APPLIED SURFACE SCIENCE
Volume 359, Issue -, Pages 82-88Publisher
ELSEVIER
DOI: 10.1016/j.apsusc.2015.09.247
Keywords
Silver nanoparticle; Bovine serum albumin; Discrete conjugate; Fluorescence quenching; Conformational change
Categories
Funding
- National Natural Science Foundation of China [21171086, 81160213]
- Inner Mongolia Grassland Talent [108-108038]
- Inner Mongolia Autonomous Region Natural Science Foundation [2013MS1121, 2015MS0806]
- Inner Mongolia Agricultural University [211-109003, 211-206038]
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The nanostructures formed by inorganic nanoparticles together with organic molecules especially biomolecules have attracted increasing attention from both industries and researching fields due to their unique hybrid properties. In this paper, we systemically studied the interactions between amphiphilic polymer coated silver nanoparticles and bovine serum albumins by employing the fluorescence quenching approach in combination with the Stern-Volmer and Hill equations. The binding affinity was determined to 1.30 x 10(7) M-1 and the interaction was spontaneously driven by mainly the van der Waals force and hydrogen-bond mediated interactions, and negatively cooperative from the point of view of thermodynamics. With the non-uniform coating of amphiphilic polymer, the silver nanoparticles can form protein coronas which can become discrete protein-nanoparticle conjugates when controlling their molar ratios of mixing. The protein's conformational changes upon binding nanoparticles was also studied by using the three-dimensional fluorescence spectroscopy. (C) 2015 Elsevier B.V. All rights reserved.
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