Journal
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
Volume 38, Issue -, Pages 206-211Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.msec.2014.02.001
Keywords
Carbon nanoparticle; Apyrase; Fluorescence anisotropy; Aptamer; Sensor
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Funding
- National Natural Science Foundation of China [21345006, 21275131]
- Physical Chemistry Institute Foundation of Zhejiang Normal University [ZJHX201315]
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Carbon nanomaterial combined with aptamer has been developed as an efficient bioanalytical method in sensor design. Herein, depending on carbon nanoparticle (cCNP)-enhanced fluorescence anisotropy (FA), a novel aptamer-based sensor (aptasensor) enabling signal-amplification and real-time detection of apyrase is reported. The foundation of our sensor design based on ATP-aptamer(P) can be adsorbed on the surface of cCNPs, resulting in the increase of FA due to the mass of cCNPs, and P-ATP complex has weak binding ability to cCNPs with minimal change of FA. Apyrase, being an integral membrane protein, can hydrolyze ATP and make P-ATP complex disassemble, and thus lead to the increasing of FA Therefore, this approach is demonstrated to be a novel candidate for the detection of apyrase, with high sensitivity and selectivity. The linear dynamic range for the concentrations of apyrase is between 0.1 and 0.5 U/mu L along with a detection limit of 0.05 U/mu L. Furthermore, these results indicated that our design is a flexible and sensitive method for biomolecule analysis, which makes it promising for practical biomolecule analyses. (C) 2014 Elsevier B.V. All rights reserved.
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