Journal
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
Volume 42, Issue -, Pages 70-77Publisher
ELSEVIER
DOI: 10.1016/j.msec.2014.04.042
Keywords
Primary osteoblast; Differentiation; Gold nanoparticles; Runt-related transcription factor 2; Extracellular signal-regulated kinases
Categories
Funding
- National Natural Science Foundation of China [21271059, 20971034]
- Research Fund for the Doctoral Program of Higher Education of China [20111301110004]
- Hundred Excellent Innovation Talents Supporting Project of Hebei Province [BR2-201]
- Training Program for Innovative Research Team and Leading Talent in Hebei Province University [LJRC024]
- Administrative Bureau of Traditional Chinese Medicine of Guangdong Province [20131260]
- Doctor Scientific Start-up Fund Projects of Guangdong Medical College [B2012007]
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Gold nanoparticles (AuNPs) have shown great promise for a variety of applications, including chemistry, biology, and medicine. Recently, AuNPs have found promising applications in cartilage and bone repair. However, to realize the above promised applications, more work needs to be carried out to clarify the interactions between biological systems and AuNPs. In the present study, primary osteoblasts were used to evaluate the biocompatibility of 20-nm and 40-nm AuNPs, including morphology, proliferation, differentiation, gene and protein expression, and the underlying mechanisms. The results demonstrated that AuNPs were taken up by osteoblasts and aggregated in perinuclear compartment and vescular structures, but no morphological changes were observed. AuNPs could significantly promote the proliferation of osteoblasts, enhance the ALP activities, and increase the number of bone nodules and calcium content in vitro. In addition, the expression of BMP-2, Runx-2, OCN and Col-1 was remarkably up-regulated in the presence of AuNPs. It is noteworthy that 20-nm AuNPs are more potent than 40-nm AuNPs in regulating osteoblast activities. Besides, AuNPs increased the level of ERR phosphorylation/total ERR, suggesting the activation of ERK/MAPK pathway is involved in above activities. In conclusion, AuNPs exhibited great biocompatibility with osteoblasts, and have tremendous potential to be used as drug and/or gene delivery carrier for bone and tissue engineering in the future. (C) 2014 Elsevier B.V. All rights reserved.
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